"Game Over" sound is looping

Link to the project or level: (Game Lab - Code.org)

What I expect to happen:
I want the “Game Over” sound to play just once when the sprite goes off the screen

What actually happens:
The sound loops (sounds like a crazy echo) because the draw loop continues iterating

What I’ve tried:
I have moved the play sound block around and tried the stop sound block

it seems like you’ve resolved your issue however you didn’t account for stopping the player from jumping after the game has finished allowing them to keep playing even though they died

// Variables
var score = -1;

// Create Sprites
var platform = createSprite(200, 200);
platform.setAnimation("platform");
platform.velocityY = 1;
platform.y = 0;
platform.x = randomNumber(50, 350);
var platform2 = createSprite(200, 200);
platform2.setAnimation("platform");
platform2.velocityY = 1;
platform2.y = 200;
platform2.x = randomNumber(50, 350);
platform.visible = false;
platform2.visible = false;
var star1 = createSprite(randomNumber(10, 390), randomNumber(-30, -100));
star1.setAnimation("star");
star1.velocityY = 3;
star1.visible = false;
var star2 = createSprite(randomNumber(10, 390), randomNumber(-30, -100));
star2.setAnimation("star");
star2.velocityY = 3;
star2.visible = false;
var player = createSprite(200, 0);
player.setAnimation("alien");
player.visible = false;
var isOver = false;
function draw() {
  // draw the background
  if (score > 25) {
    background2();
  } else if ((score > -1)) {
    background1();
  } else {
    startBackground();
  }
  if (keyWentDown("space") && !isOver) {
    score = score + 1;
    platform.visible = true;
    platform2.visible = true;
    star1.visible = true;
    star2.visible = true;
    player.visible = true;
    player.y = 0;
  }
  showScore();
  // update the sprites
  loopPlatforms();
  loopItems();
  playerFall();
  playerControl();
  playerLands();
  collectItems();
  
  drawSprites();
  gameOver();
}

// Functions
function background1() {
  background("darkBlue");
  noStroke();
  fill("yellow");
  ellipse(randomNumber(0, 400), randomNumber(0, 400), 3, 3);
  ellipse(randomNumber(0, 400), randomNumber(0, 400), 3, 3);
  ellipse(340, 50, 60, 60);
  fill("darkBlue");
  ellipse(320, 30, 60, 60);
}
function background2() {
  background("coral");
  noStroke();
  fill("yellow");
  ellipse(randomNumber(0, 400), randomNumber(0, 400), 3, 3);
  ellipse(randomNumber(0, 400), randomNumber(0, 400), 3, 3);
  ellipse(340, 50, 60, 60);
  fill("darkBlue");
  ellipse(320, 30, 60, 60);
}
function startBackground() {
  background("white");
  fill("black");
  textSize(40);
  text("Platform Jumper!!!",25, 200);
  textSize(20);
  text("Use the arrows to move the alien ",25, 250);
  text("and collect the stars.", 25, 275);
  fill("red");
  textSize(30);
  text("Press 'space' to start!", 25, 315);
}
function showScore() {
  fill("white");
  textSize(20);
  text("Score: ",10, 10, 80, 20);
  text(score, 70, 25);
}
function loopPlatforms() {
if (platform.y > 400) {
    platform.y = 0;
    platform.x = randomNumber(50, 350);
  }
if (platform2.y > 400) {
  platform2.y = 0;
  platform2.x = randomNumber(50, 350);
}
    
}
function loopItems() {
if (star1.y > 400) {
    star1.y = randomNumber(-30, -0100);
    star1.x = randomNumber(10, 390);
  }
if (star2.y > 400) {
    star2.y = randomNumber(-30, -100);
    star2.x = randomNumber(10, 390);
  }
}
function playerFall() {
  player.velocityY = player.velocityY + 0.1;
}
function playerControl() {
  if (keyDown("up")) {
    player.velocityY = player.velocityY - 0.7;
  }
  if (keyDown("left")) {
    player.x = player.x - 3;
  }
  if (keyDown("right")) {
    player.x = player.x + 3;
  }
}
function playerLands() {
  player.collide(platform);
  player.collide(platform2);
}
function collectItems() {
  if (player.isTouching(star1)) {
    score = score + 1;
    star1.y = randomNumber(-30, -0100);
    star1.x = randomNumber(10, 390);
    playSound("sound://category_achievements/lighthearted_bonus_objective_2.mp3", false);
  }
  if (player.isTouching(star2)) {
    score = score + 1;
    star2.y = randomNumber(-30, -0100);
    star2.x = randomNumber(10, 390);
    playSound("sound://category_achievements/lighthearted_bonus_objective_2.mp3", false);
  }
  
}
function gameOver() {
  if (player.y > 400) {
    background("black");
    if (!isOver) {
      playSound("sound://category_male_voiceover/mission_failed_male.mp3", false);
      isOver = !isOver;
    }
    
  }
}

here is my revised code hope this helps